Anti-HNRNPL antibody produced in mouse
SIGMA/SAB1405954 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to HNRNPL on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of HNRPL expression in transfected 293T cell line by HNRPL polyclonal antibody. Lanes Lane 1: HNRPL transfected lysate (61.38 kDa). Lane 2: Non-transfected lysate.
Western Blotting HNRNPL polyclonal antibody. Western Blot analysis of HNRNPL expression in A-431.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~60.2 kDa |
| NCBI accession no. | BC069184   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P14866 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| General description: | Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | HNRNPL (AAH69184.1, 1 a.a. ~ 558 a.a) full-length human protein. Sequence MVKMAAAGGGGGGGRYYGGGSEGGR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |