Anti-IDH3A antibody produced in mouse
SIGMA/SAB1405983 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to IDH3A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting IDH3A polyclonal antibody. Western Blot analysis of IDH3A expression in rat brain.
Western Blotting Western Blot analysis of IDH3A expression in transfected 293T cell line by IDH3A polyclonal antibody. Lanes Lane 1: IDH3A transfected lysate (40.26 kDa). Lane 2: Non-transfected lysate.
Western Blotting IDH3A polyclonal antibody. Western Blot analysis of IDH3A expression in A-431.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~39.6 kDa |
| NCBI accession no. | NM_005530.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P50213 |
| General description: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. (provided by RefSeq) |
| Immunogen: | IDH3A (NP_005521.1, 1 a.a. ~ 366 a.a) full-length human protein. Sequence MAGPAWISKVSRLLGAFHNPKQVTR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |