Anti-MCM6 antibody produced in mouse
SIGMA/SAB1406112 - purified immunoglobulin, buffered aqueous solution
Synonym: MCG40308; Mis5; P105MCM
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of antibody to MCM6 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of antibody to MCM6 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM6. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-MCM6 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of MCM6 expression in transfected 293T cell line by MCM6 polyclonal antibody. Lanes Lane 1: MCM6 transfected lysate (90.31 kDa). Lane 2: Non-transfected lysate.
Western Blotting MCM6 polyclonal antibody. Western Blot analysis of MCM6 expression in human placenta.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~92.9 kDa |
| NCBI accession no. | NM_005915   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q14566 |
| General description: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. (provided by RefSeq) |
| Immunogen: | MCM6 (NP_005906.2, 1 a.a. ~ 821 a.a) full-length human protein. Sequence MDLAAAAEPGAGSQHLEVRDEVAEK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |