Anti-RAD51 antibody produced in mouse
SIGMA/SAB1406364 - purified immunoglobulin, buffered aqueous solution
Synonym: BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RECA
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of antibody to RAD51 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 μg/mL]
Western Blotting RAD51 polyclonal antibody. Western Blot analysis of RAD51 expression in Raw 264.7.
Western Blotting RAD51 polyclonal antibody. Western Blot analysis of RAD51 expression in Jurkat.
Western Blotting RAD51 polyclonal antibody. Western Blot analysis of RAD51 expression in human colon.
Western Blotting RAD51 polyclonal antibody. Western Blot analysis of RAD51 expression in NIH/3T3.
Western Blotting RAD51 polyclonal antibody. Western Blot analysis of RAD51 expression in PC-12.
Western Blotting Western Blot analysis of RAD51 expression in transfected 293T cell line by RAD51 polyclonal antibody. Lanes Lane 1: RAD51 transfected lysate (37.29 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | NM_002875   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q06609 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) Anti-RAD51 antibody produced in mouse has been used in Western blot analysis and Immunohistochemistry. |
| Biochem/physiol Actions: | RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein replication protein A (RPA) and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. RAD51 specifically repairs double stranded breaks in the DNA. Upregulation of RAD51 is observed in a number of tumors and indicates poor prognosis. Rad51 induces G0/G1 to S phase transition in cell cycle. Therefore, inhibition of RAD51 reduces the progression of cancer in cases such as cervical cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the RAD51 protein family. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) The RAD51 gene is located on human chromosome 15q15.1. |
| Immunogen: | RAD51 (NP_002866.2, 1 a.a. ~ 339 a.a) full-length human protein. Sequence MAMQMQLEANADTSVEEESFGPQPI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |