Anti-DUSP14 antibody produced in mouse
SIGMA/SAB1407100 - purified immunoglobulin, buffered aqueous solution
Synonym: MKP-L; MKP6
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to DUSP14 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of DUSP14 expression in transfected 293T cell line by DUSP14 polyclonal antibody. Lanes Lane 1: DUSP14 transfected lysate (21.78 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~22.3 kDa |
| NCBI accession no. | NM_007026.1   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | O95147 |
| Application: | Anti-DUSP14 antibody produced in mouse is suitable for indirect immunofluorescence and western blot assay. |
| Biochem/physiol Actions: | DUSP14 (Dual specificity phosphatase 14) is associated with several critical signaling pathways. It has ability to dephosphorylate both phosphotyrosine and phosphoserine/phosphothre |
| General description: | In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28 (MIM 186760), which interacts with B7-1 (CD80; MIM 112203) and B7-2 (CD86; MIM 601020) ligands on antigen-presenting cells. CD28 costimulation induces transcription of interleukin-2 (IL2; MIM 147680) and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK (e.g., MAP2K4; MIM 601335) and JNK (see MIM 601158), and the subsequent creation of AP1 transcription factor (see MIM 165160). DUSP14 is a negative regulator of CD28 signaling.[supplied by OMIM |
| Immunogen: | DUSP14 (NP_008957.1, 1 a.a. ~ 198 a.a) full-length human protein. Sequence MSSRGHSTLPRTLMAPRMISEGDIG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |