Anti-GRHL2 antibody produced in mouse
SIGMA/SAB1408000 - purified immunoglobulin, buffered aqueous solution
Synonym: BOM; DFNA28; FLJ11172; FLJ13782; MGC149294; MGC149295; TFCP2L3
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to GRHL2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of GRHL2 expression in transfected 293T cell line by GRHL2 polyclonal antibody. Lanes Lane 1: GRHL2 transfected lysate (68.75 kDa). Lane 2: Non-transfected lysate.
Western Blotting GRHL2 polyclonal antibody. Western Blot analysis of GRHL2 expression in A-431.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~71.1 kDa |
| NCBI accession no. | NM_024915.1   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q6ISB3 |
| Application: | Anti-GRHL2 antibody produced in mouse has been used in: • Immunofluorescence • Western blotting • Co-immunoprecipitation • Immunohistochemistry |
| Biochem/physiol Actions: | Grainyhead like transcription factor 2 (GRHL2) inhibits epithelial-to-mesenchymal transition, which is necessary for cell migration. It is involved in neural tube closure, epithelial morphogenesis and barrier formation during embryogenesis. It contributes to carcinogenesis. GRHL2 regulates epithelial plasticity of pancreatic ductal adenocarcinoma (PDAC) progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Grainyhead like transcription factor 2 (GRHL2) encodes a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28). Grainyhead like transcription factor 2 (GRHL2) is an epithelial regulatory factor. GRHL2 is homologous to Drosophila grainyhead. GRHL2 gene is located on human chromosome 8q22.3. |
| Immunogen: | GRHL2 (NP_079191.1, 1 a.a. ~ 625 a.a) full-length human protein. Sequence MSQESDNNKRLVALVPMPSDPPFNT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |