Anti-MRGPRX2 antibody produced in mouse
SIGMA/SAB1408259 - purified immunoglobulin, buffered aqueous solution
Synonym: MRGX2
Product Type: Chemical
Western Blotting Western Blot analysis of MRGPRX2 expression in transfected 293T cell line by MRGPRX2 polyclonal antibody. Lanes Lane 1: MRGPRX2 transfected lysate (36.3 kDa). Lane 2: Non-transfected lysate.
Flow Cytometry FACS analysis of negative control 293 cells (Black) and MRGPRX2 expressing 293 cells (Green) using MRGPRX2 purified mouse polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~37.1 kDa |
| NCBI accession no. | NM_054030   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q96LB1 |
| Biochem/physiol Actions: | Mas-related G protein-coupled receptor X2 (MRGPRX2) expressed on mast cells acts as an important receptor for pseudo-allergic responses induced by drugs. It is involved in the evolution of the human nervous system. It is also crucial to mast cells. MRGPRX2 gene mutation impairs the risk of anaphylactic actions facilitated by the MRGPRX2 receptor. |
| General description: | Mas-related G protein-coupled receptor X2 (MRGPRX2) belonging to the class A GPCR/Mas-related gene family is expressed in sensory neurons, mast cells, and keratinocytes. It has seven transmembrane (TM) α-helices. MRGPRX2 mRNA is found at a high level in the skin. It is also detected in adipose tissue, esophagus, urinary bladder, and lungs. MRGPRX2 gene with two-exon is mapped on human chromosome 11p15. |
| Immunogen: | MRGPRX2 (NP_473371.1, 1 a.a. ~ 330 a.a) full-length human protein. Sequence MDPTTPAWGTESTTVNGNDQALLLL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |