Monoclonal Anti-STIP1 antibody produced in mouse
SIGMA/SAB1408803 - clone 2E11, purified immunoglobulin, buffered aqueous solution
Synonym: HOP; IEF-SSP-3521; P60; STI1; STI1L
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 0.75 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting STIP1 monoclonal antibody, clone 2E11. Western Blot analysis of STIP1 expression in human ovarian cancer.
Western Blotting Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody, clone 2E11. Lanes Lane 1: STIP1 transfected lysate (Predicted MW: 62.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting STIP1 monoclonal antibody, clone 2E11. Western Blot analysis of STIP1 expression in MCF-7.
Western Blotting QC Western Blot detection against Immunogen (85.25 kDa).
ELISA Detection limit for recombinant GST tagged STIP1 is 0.1 ng/mL as a capture antibody.
Flow Cytometry FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.
Flow Cytometry FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained A-431 cells (Black) as negative control.
Flow Cytometry FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.
Flow Cytometry FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained TOV-21G cells (Black) as negative control.
Flow Cytometry FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 85.25 kDa |
| NCBI accession no. | BC002987   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P31948 |
| General description: | STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM |
| Immunogen: | STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEQVNELKEKGNKALSVGNIDDALQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |