Monoclonal Anti-NKX2-5 antibody produced in mouse
SIGMA/SAB1408911 - clone 3C1, purified immunoglobulin, buffered aqueous solution
Synonym: CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to NKX2-5 on HeLa cell. [antibody concentration 15 μg/mL]
Western Blotting Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody, clone 3C1. Lanes Lane 1: NKX2-5 transfected lysate (34.9 kDa). Lane 2: Non-transfected lysate.
Immunoprecipitation Immunoprecipitation of NKX2-5 transfected lysate using anti-NKX2-5 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with NKX2-5 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 40.04 kDa |
| NCBI accession no. | NM_004387   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P52952 |
| General description: | Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called ′tinman′ is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM |
| Immunogen: | NKX2-5 (NP_004378, 1 a.a. ~ 130 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MFPSPALTPTPFSVKDILNLEQQQR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |