Monoclonal Anti-HGD antibody produced in mouse
SIGMA/SAB1409185 - clone 1F1, purified immunoglobulin, buffered aqueous solution
Synonym: AKU; HGO
Product Type: Chemical
Western Blotting Western Blot analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody, clone 1F1. Lanes Lane 1: HGD transfected lysate (50 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (61.71 kDa).
Immunoprecipitation Immunoprecipitation of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HGD rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged HGD is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 61.71 kDa |
| NCBI accession no. | BC020792   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q93099 |
| General description: | Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26. (provided by RefSeq) |
| Immunogen: | HGD (AAH20792, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAELKYISGFGNECSSEDPRCPGSL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |