Anti-HK1 antibody produced in mouse
SIGMA/SAB1409191 - purified immunoglobulin, buffered aqueous solution
Synonym: HK1-ta; HK1-tb; HK1-tc; HKI; HXK1
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to HK1 on HeLa cell. [antibody concentration 20 μg/mL]
Western Blotting HK1 polyclonal antibody. Western Blot analysis of HK1 expression in human spleen.
Western Blotting Western Blot analysis of HK1 expression in transfected 293T cell line by HK1 polyclonal antibody. Lane1:HK1 transfected lysate (100.76 kDa). Lane 2: Non-transfected lysate.
Immunoprecipitation Immunoprecipitation of HK1 transfected lysate using anti-HK1 mouse polyclonal antibody and Protein A Magnetic Bead , and immunoblotted with HK1 purified rabbit polyclonal antibody (D01P) .
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 102.2 kDa |
| NCBI accession no. | NM_033496.1   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1 μg/mL | |
| UniProt accession no. | P19367 |
| General description: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. (provided by RefSeq) |
| Immunogen: | HK1 (NP_277031.1, 1 a.a. ~ 916 a.a) full-length human protein. Sequence MDCEHSLSLPCRGAEAWEIGIDKYL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |