Monoclonal Anti-PI3 antibody produced in mouse
SIGMA/SAB1409445 - clone 2G20, purified immunoglobulin, buffered aqueous solution
Synonym: ESI; MGC13613; SKALP; WAP3; WFDC14; cementoin
Product Type: Chemical
Western Blotting Western Blot analysis of PI3 expression in transfected 293T cell line by PI3 monoclonal antibody, clone 2G20. Lanes Lane 1: PI3 transfected lysate (Predicted MW: 12.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (38.61 kDa).
Immunoprecipitation Immunoprecipitation of PI3 transfected lysate using anti-PI3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PI3 monoclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G20, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 38.61 kDa |
| NCBI accession no. | BC010952   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P19957 |
| General description: | This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. (provided by RefSeq) |
| Immunogen: | PI3 (AAH10952, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRASSFLIVVVFLIAGTLVLEAAVT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |