Monoclonal Anti-CSNK1G1 antibody produced in mouse
SIGMA/SAB1409452 - clone 3F10, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Enhanced Validation-RNAi Western blot analysis of CSNK1G1 over-expressed 293 cell line, cotransfected with CSNK1G1 Validated Chimera RNAi. Blot probed with CSNK1G1 monoclonal antibody, clone 3F10. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting Western Blot analysis of CSNK1G1 expression in transfected 293T cell line by CSNK1G1 monoclonal antibody, clone 3F10. Lanes Lane 1: CSNK1G1 transfected lysate (48.511 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3F10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 36.74 kDa |
| NCBI accession no. | BC017236   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9HCP0 |
| General description: | Casein kinase I is the most abundant serine/threonine kinase in eukaryotic cell extracts. Multiple isoforms of the enzyme exist. The gamma-1 isoform is involved in growth and morphogenesis of eukaryotic cells.[supplied by OMIM |
| Immunogen: | CSNK1G1 (AAH17236, 293 a.a. ~ 393 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KKGYTFDYAYDWVGRPIPTPVGSVH |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |