Monoclonal Anti-PPP3CC antibody produced in mouse
SIGMA/SAB1409493 - clone 4D1, purified immunoglobulin, buffered aqueous solution
Synonym: CALNA3
Product Type: Chemical
Western Blotting Western Blot analysis of PPP3CC expression in transfected 293T cell line by PPP3CC monoclonal antibody, clone 4D1. Lanes Lane 1: PPP3CC transfected lysate (Predicted MW: 58.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (34.65 kDa).
ELISA Detection limit for recombinant GST tagged PPP3CC is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 34.65 kDa |
| NCBI accession no. | NM_005605   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P48454 |
| General description: | Calmodulin-dependent protein phosphatase, calcineurin, is involved in a wide range of biologic activities, acting as a Ca(2+)-dependent modifier of phosphorylation status. In testis, the motility of the sperm is thought to be controlled by cAMP-dependent phosphorylation and a unique form of calcineurin appears to be associated with the flagellum. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. At least 3 genes, calcineurin A-alpha (CALNA1; MIM 114105), calcineurin A-beta (CALNA2; MIM 114106), and calcineurin A-gamma (CALNA3), have been cloned for the catalytic subunit. These genes have been identified in humans, mice, and rats, and are highly conserved between species (90 to 95% amino acid identity).[supplied by OMIM |
| Immunogen: | PPP3CC (NP_005596.2, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSGRRFHLSTTDRVIKAVPFPPTQR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |