Monoclonal Anti-CDKN2AIP antibody produced in mouse
SIGMA/SAB1409502 - clone 1F7, purified immunoglobulin, buffered aqueous solution
Synonym: CARF; FLJ20036
Product Type: Chemical
Western Blotting Western Blot analysis of CARF expression in transfected 293T cell line by CARF monoclonal antibody, clone 1F7. Lanes Lane 1: CARF transfected lysate (61.1 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of CARF over-expressed 293 cell line, cotransfected with CARF Validated Chimera RNAi. Blot probed with CARF monoclonal antibody, clone 1F7. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (89.43 kDa).
ELISA Detection limit for recombinant GST tagged CARF is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 89.43 kDa |
| NCBI accession no. | BC022270   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q8N187 |
| Immunogen: | CARF (AAH22270, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAQEVSEYLSQNPRVAAWVEALRCD |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |