Monoclonal Anti-PURA antibody produced in mouse
SIGMA/SAB1409585 - clone 1C10, purified immunoglobulin, buffered aqueous solution
Synonym: PUR-ALPHA; PUR1; PURALPHA
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PURA on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting PURA monoclonal antibody, clone 1C10. Western Blot analysis of PURA expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged PURA is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 37.84 kDa |
| NCBI accession no. | NM_005859   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q00577 |
| General description: | This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. (provided by RefSeq) |
| Immunogen: | PURA (NP_005850, 183 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TQGQTIALPAQGLIEFRDALAKLID |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |