Monoclonal Anti-S100A7 antibody produced in mouse
SIGMA/SAB1409618 - clone 3A5, purified immunoglobulin, buffered aqueous solution
Synonym: PSOR1; S100A7c
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to S100A7 on HeLa cell. [antibody concentration 20 μg/mL]
Western Blotting Western Blot analysis of S100A7 expression in transfected 293T cell line by S100A7 monoclonal antibody, clone 3A5. Lanes Lane 1: S100A7 transfected lysate (11.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.85 kDa).
ELISA Detection limit for recombinant GST tagged S100A7 is approximately 10 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen 36.85 kDa |
| NCBI accession no. | BC034687   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P31151 |
| General description: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. (provided by RefSeq) |
| Immunogen: | S100A7 (AAH34687.1, 1 a.a. ~ 101 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSNTQAERSIIGMIDMFHKYTRRDD |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |