Monoclonal Anti-UBTF antibody produced in mouse
SIGMA/SAB1409786 - clone 2C6, purified immunoglobulin, buffered aqueous solution
Synonym: NOR-90; UBF
Product Type: Chemical
Western Blotting UBTF monoclonal antibody, clone 2C6 Western Blot analysis of UBTF expression in HepG2.
Western Blotting UBTF monoclonal antibody, clone 2C6. Western Blot analysis of UBTF expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged UBTF is approximately 30ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 36.74 kDa |
| NCBI accession no. | NM_014233   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P17480 |
| General description: | Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or ′TAFs′). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM |
| Immunogen: | UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PPAATNSSKKMKFQGEPKKPPMNGY |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |