Monoclonal Anti-PCAF antibody produced in mouse
SIGMA/SAB1409953 - clone 5E5, purified immunoglobulin, buffered aqueous solution
Synonym: CAF; P; P/CAF; PCAF
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PCAF on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting PCAF monoclonal antibody, clone 5E5. Western Blot analysis of PCAF expression in human thyroid(diffuse hyperplasia).
Western Blotting PCAF monoclonal antibody, clone 5E5. Western Blot analysis of PCAF expression in human uterus myoma.
Western Blotting PCAF monoclonal antibody, clone 5E5. Western Blot analysis of PCAF expression in Raw 264.7.
Western Blotting PCAF monoclonal antibody, clone 5E5. Western Blot analysis of PCAF expression in A-431.
Western Blotting PCAF monoclonal antibody, clone 5E5. Western Blot analysis of PCAF expression in C32.
Western Blotting PCAF monoclonal antibody, clone 5E5 Western Blot analysis of PCAF expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged PCAF is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5E5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 36.63 kDa |
| NCBI accession no. | BC060823   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. (provided by RefSeq) |
| Immunogen: | PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LEEEVYSQNSPIWDQDFLSASSRTS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |