Anti-NUDT5 antibody produced in rabbit
SIGMA/SAB1410166 - purified immunoglobulin, buffered aqueous solution
Synonym: YSA1; YSA1H; hYSAH1
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to NUDT5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NUDT5 expression in transfected 293T cell line by NUDT5 polyclonal antibody. Lanes Lane 1: NUDT5 transfected lysate (24.30 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 24.3 kDa |
| NCBI accession no. | NM_014142.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q9UKK9 |
| Biochem/physiol Actions: | NUDT5 (Nudix, nucleoside diphosphate linked moiety X-type motif 5) is mainly involved in maintaining the intracellular level of ADP-ribose by hydrolyzing ADP-ribose (ADPR) to AMP and ribose 5′-phosphate. It also restricts non-enzymatic ADP-ribosylation. Its N-terminal antiparallel β-sheet plays an important role in dimerization, which is essential for substrate recognition and enzymatic activity. NUDT5 has also been predicted for altered cell proliferation. It has been suggested that cells with suppressed NUDT5 shows delayed cell cycle G1 phase. Thus, it has been concluded that NUDT5 may involve in cell growth and cell cycle proliferation by regulating G1-S transition in mammalian cells. |
| General description: | Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan, 1999 [PubMed 10373642]).[supplied by OMIM |
| Immunogen: | NUDT5 (NP_054861.2, 1 a.a. ~ 219 a.a) full-length human protein. Sequence MESQEPTESSQNGKQYIISEELISE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |