Anti-RNF168 antibody produced in rabbit
SIGMA/SAB1410293 - purified immunoglobulin, buffered aqueous solution
Synonym: FLJ35794
Product Type: Chemical
Western Blotting Western Blot analysis of RNF168 expression in transfected 293T cell line by RNF168 polyclonal antibody. Lanes Lane 1: RNF168 transfected lysate (65.00 kDa). Lane 2: Non-transfected lysate.
Western Blotting RNF168 rabbit polyclonal antibody. Western Blot analysis of RNF168 expression in human liver.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 65 kDa |
| NCBI accession no. | NM_152617.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1 μg/mL |
| UniProt accession no. | Q8IYW5 |
| Biochem/physiol Actions: | Ring finger protein 168 (RNF168) plays a vital role in chromatin modification by stimulating histone ubiquitination. In addition, it also initiates DNA damage response to double-strand breaks (DSBs). Mutation in the gene leads to RIDDLE (radiosensitivity, immunodeficiency, dysmorphic features and learning difficulties) syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ring finger protein 168 (RNF168) is a novel chromatin-associated ubiquitin ligase, encoded by the gene mapped to human chromosome 3q29. RNF168 is characterized with a RING(really interesting new gene) domain and two motifs interacting with ubiquitin (MIU). |
| General description: | The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]). |
| Immunogen: | RNF168 (NP_689830.2, 1 a.a. ~ 571 a.a) full-length human protein. Sequence MALPKDAIPSLSECQCGICMEILVE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |