Anti-DFFB antibody produced in rabbit
SIGMA/SAB1410294 - purified immunoglobulin, buffered aqueous solution
Synonym: CAD; CPAN; DFF-40; DFF2; DFF40
Product Type: Chemical
Western Blotting Western Blot analysis of DFFB expression in transfected 293T cell line by DFFB polyclonal antibody. Lanes Lane 1: DFFB transfected lysate (39.10 kDa). Lane 2: Non-transfected lysate.
Western Blotting DFFB rabbit polyclonal antibody. Western Blot analysis of DFFB expression in mouse liver.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 39.1 kDa |
| NCBI accession no. | NM_004402   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1 μg/mL |
| UniProt accession no. | O76075 |
| General description: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. (provided by RefSeq) |
| Immunogen: | DFFB (NP_004393.1, 1 a.a. ~ 338 a.a) full-length human protein. Sequence MLQKPKSVKLRALRSPRKFGVAGRS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |