Anti-ALOX5 antibody produced in rabbit
SIGMA/SAB1410449 - purified immunoglobulin, buffered aqueous solution
Synonym: 5-LO; 5-LOX; 5LPG; LOG5; MGC163204
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to ALOX5 on HeLa cell. [antibody concentration 30 μg/mL]
Western Blotting ALOX5 rabbit polyclonal antibody. Western Blot analysis of ALOX5 expression in C32.
Western Blotting Western Blot analysis of ALOX5 expression in transfected 293T cell line by ALOX5 polyclonal antibody. Lanes Lane 1: ALOX5 transfected lysate (78.00 kDa). Lane 2: Non-transfected lysate.
Western Blotting ALOX5 rabbit polyclonal antibody. Western Blot analysis of ALOX5 expression in mouse spleen.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 78 kDa |
| NCBI accession no. | NM_000698.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P09917 |
| Biochem/physiol Actions: | The gene ALOX5 (arachidonate 5-lipoxygenase) encodes a lipoxygenase that catalyzes the conversion of arachidonic acid (AA) and other fatty acids to bioactive compounds such as 5(S)-hydroxyeicosatetraen |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene encoding arachidonate 5-lipoxygenase (ALOX5) is localized on human chromosome 10q11.21 and has 13 introns. It spans a region of 71.9 kbp. The ALOX5 protein consists of 673 amino acids and has a molecular weight of 77.9 kDa. It possesses two domains, a regulatory C2-like domain that binds to calcium and a carboxy-terminal catalytic domain. ALOX5 is expressed in B cells, dendritic cells, mast cells, macrophages and polymorphonuclear leukocytes. |
| General description: | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans- |
| Immunogen: | ALOX5 (NP_000689.1, 1 a.a. ~ 674 a.a) full-length human protein. Sequence MPSYTVTVATGSQWFAGTDDYIYLS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |