Anti-NEK3 antibody produced in rabbit
SIGMA/SAB1410835 - purified immunoglobulin, buffered aqueous solution
Synonym: HSPK36; MGC29949
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to NEK3 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of NEK3 expression in transfected 293T cell line by NEK3 polyclonal antibody. Lanes Lane 1: NEK3 transfected lysate (57.70 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 57.7 kDa |
| NCBI accession no. | NM_002498.1   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P51956 |
| General description: | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene |
| Immunogen: | NEK3 (NP_002489.1, 1 a.a. ~ 506 a.a) full-length human protein. Sequence MDDYMVLRMIGEGSFGRALLVQHES |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |