Anti-NUMA1 antibody produced in rabbit
SIGMA/SAB1410850 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to NUMA1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting NUMA1 rabbit polyclonal antibody. Western Blot analysis of NUMA1 expression in human spleen.
Western Blotting Western Blot analysis of NUMA1 expression in transfected 293T cell line by NUMA1 polyclonal antibody. Lanes Lane 1: NUMA1 transfected lysate (133.20 kDa). Lane 2: Non-transfected lysate.
Western Blotting NUMA1 rabbit polyclonal antibody. Western Blot analysis of NUMA1 expression in mouse spleen.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 133.2 kDa |
| NCBI accession no. | BC013023   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q14980 |
| Biochem/physiol Actions: | NUMA1 (Nuclear mitotic apparatus protein 1) is involved in several chromosomal maintenance activities as an essential mitotic component including spindle positioning, proper organization and maintenance of the mitotic spindle structure. During proper positioning and elongation of mitotic spindle, NUMA1, positioned above two spindle poles, directly binds to the conserved proteins (LGN/Gαi1-3) and dynein to the cell cortex. NUMA1-LGN/Gαi1-3 binding finally enhances cortical levels of NuMA/dynein, which is highly essential for robust spindle elongation. |
| General description: | NUMA1 (Nuclear mitotic apparatus protein 1) is a coiled-coil protein consisting of a globular head and tail domain, which is linked by a large coiled-coil region. Its tail domain is composed of microtubule, LGN binding sites, and a nuclear localization signal (NLS). |
| Immunogen: | NUMA1 (AAH13023.1, 1 a.a. ~ 1193 a.a) full-length human protein. Sequence MAATSKEVARLETLVRKAGEQQETA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |