Anti-MECR antibody produced in rabbit
SIGMA/SAB1410898 - purified immunoglobulin, buffered aqueous solution
Synonym: CGI-63; FASN2B; NRBF1
Product Type: Chemical
Western Blotting Western Blot analysis of MECR expression in transfected 293T cell line by MECR polyclonal antibody. Lanes Lane 1: MECR transfected lysate (40.50 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 40.5 kDa |
| NCBI accession no. | NM_016011.2   |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1 μg/mL |
| UniProt accession no. | Q9BV79 |
| Biochem/physiol Actions: | MECR (Mitochondrial trans-2-enoyl-CoA reductase) plays a vital role in the intracellular signaling pathways of fatty acid elongation cycle pathway. In the nuclei, it catalyzes reduction reaction of trans-2-enoyl thioesters to the corresponding saturated acyl thioesters in the presence of NADPH. It has also been reported that MECR acts as a component of the mitochondrial FAS II (fatty acid synthesis II) pathway and enhances the PPARa-dependent luciferase activity in HeLa cells. |
| General description: | MECR (Mitochondrial trans-2-enoyl-CoA reductase) is a mitochondrial localized protein, also referred to as nuclear receptor binding factor-1 (NRBF-1). It consists of N-terminal mitochondrial targeting sequence, which plays an essential role for its mitochondrial localization. It was firstly identified as a binding protein of the nuclear hormone receptor peroxisome proliferator-activated receptor (PPARα). |
| Immunogen: | MECR (NP_057095.2, 1 a.a. ~ 373 a.a) full-length human protein. Sequence MWVCSTLWRVRTPARQWRGLLPASG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |