Anti-PDGFA antibody produced in rabbit
SIGMA/SAB1410937 - purified immunoglobulin, buffered aqueous solution
Synonym: PDGF-A; PDGF1
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between PDGFA and PDGFRA. HeLa cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PDGFRA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between PDGFA and PLCG1. Huh7 cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of PDGFA expression in transfected 293T cell line by PDGFA polyclonal antibody. Lanes Lane 1: PDGFA transfected lysate (24.00 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 24 kDa |
| NCBI accession no. | NM_002607   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | proximity ligation assay: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P04085 |
| General description: | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. (provided by RefSeq) |
| Immunogen: | PDGFA (NP_002598.4, 1 a.a. ~ 211 a.a) full-length human protein. Sequence MRTLACLLLLGCGYLAHVLAEEAEI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |