Anti-CYCS antibody produced in rabbit
SIGMA/SAB1410987 - purified immunoglobulin, buffered aqueous solution
Synonym: CYC; HCS
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between CYCS and CASP9. HeLa cells were stained with anti-CYCS rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of CYCS expression in transfected 293T cell line by CYCS polyclonal antibody. Lanes Lane 1: CYCS transfected lysate (11.70 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 11.7 kDa |
| NCBI accession no. | NM_018947   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | proximity ligation assay: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P99999 |
| General description: | This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. (provided by RefSeq) |
| Immunogen: | CYCS (NP_061820.1, 1 a.a. ~ 105 a.a) full-length human protein. Sequence MGDVEKGKKIFIMKCSQCHTVEKGG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |