Anti-PPT1 antibody produced in rabbit
SIGMA/SAB1411057 - purified immunoglobulin, buffered aqueous solution
Synonym: CLN1; INCL; PPT
Product Type: Chemical
Western Blotting Western Blot analysis of PPT1 expression in transfected 293T cell line by PPT1 polyclonal antibody. Lanes Lane 1: PPT1 transfected lysate (34.20 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 34.2 kDa |
| NCBI accession no. | NM_000310   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1 μg/mL |
| UniProt accession no. | P50897 |
| Biochem/physiol Actions: | Palmitoyl-protein thioesterase 1 (PPT1) functions to remove fatty acid associated with lipid-modified proteins from the cysteine residues. Mutations in the CLN1 or the PPT1 gene is implicated in the cognitive and motor decline disorder in infants called the infantile neuronal ceroid lipofuscinosis. PPT1 is glycosylated and is essential for its functionality. |
| General description: | Palmitoyl-protein thioesterase 1 (PPT1) is a lysosomal enzyme 1) It is mapped to human chromosome 1p34.2 and has eight exons. It is depalmitoylation enzyme with a signal sequence and is targeted to lysosomes through mannose 6-phosphate receptor pathway. It is also localized in axons, synaptic vesicles and synaptosomes of neurons. |
| Immunogen: | PPT1 (NP_000301.1, 1 a.a. ~ 306 a.a) full-length human protein. Sequence MASPGCLWLLAVALLPWTCASRALQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |