Anti-PRKAG1 antibody produced in rabbit
SIGMA/SAB1411069 - purified immunoglobulin, buffered aqueous solution
Synonym: AMPKG; MGC8666
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to PRKAG1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PRKAG1 expression in transfected 293T cell line by PRKAG1 polyclonal antibody. Lanes Lane 1: PRKAG1 transfected lysate (28.30 kDa). Lane 2: Non-transfected lysate.
Western Blotting PRKAG1 rabbit polyclonal antibody. Western Blot analysis of PRKAG1 expression in A-431.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 28.3 kDa |
| NCBI accession no. | NM_212461   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P54619 |
| General description: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. (provided by RefSeq) |
| Immunogen: | PRKAG1 (NP_997626.1, 1 a.a. ~ 247 a.a) full-length human protein. Sequence MLTITDFINILHRYYKSALVQIYEL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |