Anti-RIOK2 antibody produced in rabbit
SIGMA/SAB1411074 - purified immunoglobulin, buffered aqueous solution
Synonym: FLJ11159
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to RIOK2 on HeLa cell. [antibody concentration 30 μg/mL]
Western Blotting Western Blot analysis of RIOK2 expression in transfected 293T cell line by RIOK2 polyclonal antibody. Lanes Lane 1: RIOK2 transfected lysate (63.30 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 63.3 kDa |
| NCBI accession no. | BC000953.2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q9BVS4 |
| Biochem/physiol Actions: | Serine/threonine-protein kinase RIO2 (RIOK2) is a nonribosomal factor required for normal ribosomal RNA biogenesis and cell cycle progression. It is necessary for processing of 20S pre-rRNA to the 18S rRNA in 40S ribosomal subunit synthesis. RIOK2 is also a ribosomal assembly factor that prevents premature translation initiation on the small (40S) subunit. It is needed for proper mitotic progression, regulates metaphase-anaphase transition and is a substrate of polo-like kinase 1 (Plk1) which is phosphorylated by Plk1 at the Ser-335, Ser-380 and Ser-548 residues. |
| General description: | Serine/threonine-protein kinase RIO2 (RIOK2) belongs to the RIO kinase family of protein kinases and the gene encoding this protein is located on human chromosome 5. |
| Immunogen: | RIOK2 (AAH00953.1, 1 a.a. ~ 552 a.a) full-length human protein. Sequence MGKVNVAKLRYMSRDDFRVLTAVEM |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |