Anti-SMURF1 antibody produced in rabbit
SIGMA/SAB1411134 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 polyclonal antibody. Lanes Lane 1: SMURF1 transfected lysate (83.40 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 83.4 kDa |
| NCBI accession no. | NM_181349   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | proximity ligation assay: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q9HCE7 |
| General description: | This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined. (provided by RefSeq) |
| Immunogen: | SMURF1 (NP_851994.1, 1 a.a. ~ 731 a.a) full-length human protein. Sequence MSNPGTRRNGSSIKIRLTVLCAKNL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |