Anti-MRPL12 antibody produced in rabbit
SIGMA/SAB1411206 - purified immunoglobulin, buffered aqueous solution
Synonym: 5c5-2; FLJ60124; L12mt; MGC8610; MRP-L31/34; MRPL7; MRPL7/L12; RPML12
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to MRPL12 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 polyclonal antibody. Lanes Lane 1: MRPL12 transfected lysate (21.30 kDa). Lane 2: Non-transfected lysate.
Western Blotting MRPL12 rabbit polyclonal antibody. Western Blot analysis of MRPL12 expression in human kidney.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 21.3 kDa |
| NCBI accession no. | NM_002949   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P52815 |
| Biochem/physiol Actions: | MRPL12 (mitochondrial ribosomal protein) is involved in the mitochondrial translation. It directly interacts with the mitochondrial RNA polymerase (POLRMT) and triggers the mitochondrial transcriptional process. Research shows that the interaction between MRPL12 and POLRMT indicates its participation in the regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. Study shows that MRPL12 may be involved in the differentiation of colonic epithelial cells and its reduced mitochondrial activity have been shown to be linked with tumor formation. It has been reported that mutated expression of MRPL12 associates with the growth retardation, neurological deterioration and mitochondrial translation deficiency. |
| General description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. (provided by RefSeq) |
| Immunogen: | MRPL12 (NP_002940.2, 1 a.a. ~ 198 a.a) full-length human protein. Sequence MLPAAARPLWGPCLGLRAAAFRLAR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |