Anti-DAP3 antibody produced in rabbit
SIGMA/SAB1411414 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to DAP3 on HeLa cell. [antibody concentration 30 μg/mL]
Western Blotting Western Blot analysis of DAP3 expression in transfected 293T cell line by DAP3 polyclonal antibody. Lanes Lane 1: DAP3 transfected lysate (45.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting DAP3 rabbit polyclonal antibody. Western Blot analysis of DAP3 expression in Jurkat.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45.6 kDa |
| NCBI accession no. | NM_004632.2   |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P51398 |
| General description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5′ UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. (provided by RefSeq) |
| Immunogen: | DAP3 (NP_004623.1, 1 a.a. ~ 398 a.a) full-length human protein. Sequence MMLKGITRLISRIHKLDPGRFLHMG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |