Anti-NUMB antibody produced in rabbit
SIGMA/SAB1411549 - purified immunoglobulin, buffered aqueous solution
Synonym: S171
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between NUMB and APP. HeLa cells were stained with anti-NUMB rabbit purified polyclonal 1:1200 and anti-APP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of NUMB expression in transfected 293T cell line by NUMB polyclonal antibody. Lanes Lane 1: NUMB transfected lysate (14.50 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 14.5 kDa |
| NCBI accession no. | BC033824   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | proximity ligation assay: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P49757 |
| General description: | The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | NUMB (AAH33824.1, 1 a.a. ~ 135 a.a) full-length human protein. Sequence MPYPAPNVPVVGITPSQMVANVFGT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |