ANTI-AKT2 antibody produced in mouse
SIGMA/SAB1411920 - clone 1D9, purified immunoglobulin, buffered aqueous solution
Synonym: AKT2; PKBB; PKBBETA; PRKBB; RAC-BETA
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting AKT2 monoclonal antibody, clone 1D9. Western Blot analysis of AKT2 expression in PC-12.
Western Blotting AKT2 monoclonal antibody, clone 1D9 Western Blot analysis of AKT2 expression in Jurkat.
Western Blotting AKT2 monoclonal antibody, clone 1D9. Western Blot analysis of AKT2 expression in Raw 264.7.
Western Blotting AKT2 monoclonal antibody, clone 1D9. Western Blot analysis of AKT2 expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
ELISA Detection limit for recombinant GST tagged AKT2 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 35.53 kDa |
| NCBI accession no. | M95936   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | , human, |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P31751 |
| General description: | This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. (provided by RefSeq) |
| Immunogen: | AKT2 (AAA58364, 100 a.a. ~ 189 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRAIQMVANSLKQRAPGEDPMDYKC |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |