ANTI-CDK8 antibody produced in mouse
SIGMA/SAB1411939 - clone 5H4, purified immunoglobulin, buffered aqueous solution
Synonym: CDK8; K35; MGC126074; MGC126075
Product Type: Chemical
Western Blotting CDK8 monoclonal antibody, clone 5H4. Western Blot analysis of CDK8 expression in NIH/3T3.
Western Blotting CDK8 monoclonal antibody, clone 5H4. Western Blot analysis of CDK8 expression in Raw 264.7.
Western Blotting CDK8 monoclonal antibody, clone 5H4 Western Blot analysis of CDK8 expression in HeLa S3 NE.
Western Blotting CDK8 monoclonal antibody, clone 5H4. Western Blot analysis of CDK8 expression in PC-12.
Western Blotting CDK8 monoclonal antibody, clone 5H4. Western Blot analysis of CDK8 expression in K-562.
Western Blotting CDK8 monoclonal antibody, clone 5H4. Western Blot analysis of CDK8 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (35.53 kDa).
ELISA Detection limit for recombinant GST tagged CDK8 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5H4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 35.53 kDa |
| NCBI accession no. | NM_001260   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49336 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the ′Mediator-like′ protein complexes and the basal transcription machinery. (provided by RefSeq) |
| Immunogen: | CDK8 (NP_001251, 375 a.a. ~ 464 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QQQGNNHTNGTGHPGNQDSSHTQGP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |