ANTI-TOP3B antibody produced in mouse
SIGMA/SAB1412100 - clone 1H8, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ39376; TOP3B
Product Type: Chemical
Western Blotting Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody, clone 1H8. Lanes Lane 1: TOP3B transfected lysate (96.662 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (120.56 kDa).
ELISA Detection limit for recombinant GST tagged TOP3B is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1H8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 120.56 kDa |
| NCBI accession no. | BC002432   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95985 |
| General description: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. (provided by RefSeq) |
| Immunogen: | TOP3B (AAH02432, 1 a.a. ~ 862 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MKTVLMVAEKPSLAQSIAKILSRGS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |