ANTI-TNIK antibody produced in mouse
SIGMA/SAB1412107 - clone 4E4, purified immunoglobulin, buffered aqueous solution
Synonym: TNIK
Product Type: Chemical
Western Blotting Western Blot analysis of TNIK expression in transfected 293T cell line by TNIK monoclonal antibody, clone 4E4. Lanes Lane 1: TNIK transfected lysate (60.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
Immunoprecipitation Immunoprecipitation of TNIK transfected lysate using anti-TNIK monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TNIK monoclonal antibody.
ELISA Detection limit for recombinant GST tagged TNIK is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 37.73 kDa |
| NCBI accession no. | BC055427   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UKE5 |
| General description: | Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM |
| Immunogen: | TNIK (AAH55427, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MKKVTDYSSSSEESESSEEEEEDGE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |