ANTI-SP1 antibody produced in mouse
SIGMA/SAB1412220 - clone 4H6, purified immunoglobulin, buffered aqueous solution
Synonym: SP1
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SP1 monoclonal antibody, clone 4H6. Western Blot analysis of SP1 expression in HeLa S3 NE.
Western Blotting SP1 monoclonal antibody, clone 4H6. Western Blot analysis of SP1 expression in NIH/3T3.
Western Blotting SP1 monoclonal antibody, clone 4H6 Western Blot analysis of SP1 expression in IMR-32.
Western Blotting QC Western Blot detection against Immunogen (37.73 kDa).
ELISA Detection limit for recombinant GST tagged SP1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | antigen 37.84 kDa |
| NCBI accession no. | NM_138473   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P08047 |
| Biochem/physiol Actions: | Sp1 protein was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including Simian vacuolating virus 40 (SV40) early, human immunodeficiency virus-1 (HIV-1), platelet-derived growth factor subunit B (PDGF-B), Myc, c-Src etc. In addition to transcription, Sp1 function has been linked to cell growth, cancer and Huntington disease. |
| General description: | Sp1 protein was the first transcription factor to be cloned and characterized. It was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. |
| Immunogen: | SP1 (NP_612482.2, 89 a.a. ~ 198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GTGELDLTATQLSQGANGWQIISSS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |