ANTI-MAPK9 antibody produced in mouse
SIGMA/SAB1412229 - clone 3C12, purified immunoglobulin, buffered aqueous solution
Synonym: JNK-55; JNK2; JNK2A; JNK2ALPHA; MAPK9
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting MAPK9 monoclonal antibody, clone 3C12 Western Blot analysis of MAPK9 expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.07 kDa).
ELISA Detection limit for recombinant GST tagged MAPK9 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 37.07 kDa |
| NCBI accession no. | BC032539   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P45984 |
| General description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene′s mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. (provided by RefSeq) |
| Immunogen: | MAPK9 (AAH32539, 321 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ITVWYDPAEAEAPPPQIYDAQLEER |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |