ANTI-TFAP4 antibody produced in mouse
SIGMA/SAB1412283 - clone 8G6, purified immunoglobulin, buffered aqueous solution
Synonym: AP-4; TFAP4; bHLHc41
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 μg/mL]
Western Blotting Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody, clone 8G6. Lanes Lane 1: TFAP4 transfected lysate (38.87 kDa). Lane 2: Non-transfected lysate.
Western Blotting TFAP4 monoclonal antibody, clone 8G6. Western Blot analysis of TFAP4 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged TFAP4 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8G6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 36.74 kDa |
| NCBI accession no. | NM_003223   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q01664 |
| General description: | Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM |
| Immunogen: | TFAP4 (NP_003214, 93 a.a. ~ 192 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AEYIFSLEQEKTRLLQQNTQLKRFI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |