ANTI-BRAF antibody produced in mouse
SIGMA/SAB1412333 - clone 1F2, purified immunoglobulin, buffered aqueous solution
Synonym: B-RAF1; BRAF; BRAF1; FLJ95109; MGC126806
Product Type: Chemical
Western Blotting QC Western Blot detection against Immunogen (36.34 kDa).
ELISA Detection limit for recombinant GST tagged BRAF is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1F2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen 35.97 kDa |
| NCBI accession no. | NM_004333   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15056 |
| General description: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. (provided by RefSeq) |
| Immunogen: | BRAF (NP_004324, 138 a.a. ~ 231 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FQNPTDVARSNPKSPQKPIVRVFLP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |