ANTI-ARHGEF1 antibody produced in mouse
SIGMA/SAB1412387 - clone 2D2, purified immunoglobulin, buffered aqueous solution
Synonym: ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF
Product Type: Chemical
Western Blotting Western Blot analysis of ARHGEF1 expression in transfected 293T cell line by ARHGEF1 monoclonal antibody, clone 2D2. Lanes Lane 1: ARHGEF1 transfected lysate (Predicted MW: 102.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.41 kDa).
ELISA Detection limit for recombinant GST tagged ARHGEF1 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 36.41 kDa |
| NCBI accession no. | BC034013   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q92888 |
| General description: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. (provided by RefSeq) |
| Immunogen: | ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence CRPGPEGQLAATALRKVLSLKQLLF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |