ANTI-SMAD1 antibody produced in mouse
SIGMA/SAB1412594 - clone 3G4, purified immunoglobulin, buffered aqueous solution
Synonym: BSP1; JV4-1; JV41; MADH1; SMAD1
Product Type: Chemical
Immunoblotting Monoclonal Anti-SMAD1: Cat. No. SAB1412594: Antibody concentration: 1 μg/mL. Specific band of ~35.2 kDa using immunogen protein lysate.
Western Blotting QC Western Blot detection against Immunogen (35.24 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen 34.87 kDa |
| NCBI accession no. | NM_005900   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15797 |
| General description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ′mothers against decapentaplegic′ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. (provided by RefSeq) |
| Immunogen: | SMAD1 (NP_005891, 176 a.a. ~ 259 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FQQPNSHPFPHSPNSSYPNSPGSSS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |