ANTI-RPS6KB1 antibody produced in mouse
SIGMA/SAB1412626 - clone 4H4, purified immunoglobulin, buffered aqueous solution
Synonym: PS6K; RPS6KB1; S6K; S6K1; STK14A
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to RPS6KB1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting RPS6KB1 monoclonal antibody, clone 4H4 Western Blot analysis of RPS6KB1 expression in HeLa.
Western Blotting RPS6KB1 monoclonal antibody, clone 4H4. Western Blot analysis of RPS6KB1 expression in Raw 264.7.
Western Blotting RPS6KB1 monoclonal antibody, clone 4H4. Western Blot analysis of RPS6KB1 expression in NIH/3T3.
Western Blotting RPS6KB1 monoclonal antibody, clone 4H4. Western Blot analysis of RPS6KB1 expression in PC-12.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4H4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen 37.84 kDa |
| NCBI accession no. | NM_003161   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P23443 |
| General description: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. (provided by RefSeq) |
| Immunogen: | RPS6KB1 (NP_003152, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PSVLESVKEKFSFEPKIRSPRRFIG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |