Anti-EIF2AK1 antibody produced in rabbit
SIGMA/SAB2100658 - affinity isolated antibody
Synonym: Anti-Eukaryotic translation initiation factor 2-α kinase 1; Anti-HRI; Anti-KIAA1369
MDL Number: MFCD03455796
Product Type: Chemical
Immunohistochemistry Anti-EIF2AK1: Cat. No. SAB2100658: Immunohistochemistry of EIF2AK1 in Lung tissue with EIF2AK1 antibody at 5.0 μg/mL.
Immunoblotting Anti-EIF2AK1: Cat. No.SAB2100658: EIF2AK1 in Fetal Lung tissue lysate with EIF2AK1 antibody at 1.0 μg/mL.
Immunoblotting EIF2AK1: Cat. No. SAB2100658: Western blot analysis of EIF2AK1 in Human HeLa cell lysates with EIF2AK1 antibody at 1.0 μg/mL.
Immunoblotting EIF2AK1: Cat. No. SAB2100658: Western blot analysis of EIF2AK1 in Human 293T cell lysates with EIF2AK1 antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 71 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | dog, guinea pig, horse, human, mouse, rabbit, rat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9BQI3  |
| Biochem/physiol Actions: | EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3′ end slightly overlaps the 3′ end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human EIF2AK1 |
| Other Notes: | Synthetic peptide located within the following region: TCSDEFSSLRLHHNRAITHLMRSAK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |