Anti-RAD51 antibody produced in rabbit
SIGMA/SAB2101936 - affinity isolated antibody
Synonym: Anti-BRCC5; Anti-HRAD51; Anti-HsRad51; Anti-HsT16930; Anti-RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
MDL Number: MFCD02686567
Product Type: Chemical
Immunohistochemistry Anti-RAD51: Cat. No. SAB2101936: Immunohistochemistry of RAD51 in Adrenal tissue with RAD51 antibody at 4-8 μg/mL.
Immunoblotting Anti-RAD51: Cat. No.SAB2101936: RAD51 in HepG2 cell lysate with RAD51 antibody at 1.0 μg/mL.
Western Blotting Western Blot of RAD51 in Human 293T with RAD51 antibody at 1 μg/mL
Western Blotting Western Blot of RAD51 in Mouse Liver with RAD51 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 26 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, bovine, rat, guinea pig, rabbit, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q06609-2  |
| Biochem/physiol Actions: | RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human RAD51 |
| Other Notes: | Synthetic peptide located within the following region: ANDVKKLEEAGFHTVEAVAYAPKKE |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |