Anti-PLOD2 antibody produced in rabbit
SIGMA/SAB2103796 - affinity isolated antibody
Synonym: Anti-LH2; Anti-TLH
Product Type: Chemical
Immunohistochemistry PLOD2 Antibody: Cat. No. SAB2103796: Immunohistochemistry analysis of PLOD2 in Human Bronchial Epithelial Tissue with PLOD2 Antibody 5.0 μg/mL. Observed Staining: Cytoplasmic
Immunoblotting Anti-PLOD2, antibody produced in rabbit: Cat. No. SAB2103796: Immunoblotting analysis of PLOD2 in DU145 cell lysate with PLOD2 anitbody at 1 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 84 kDa |
| NCBI accession no. | NM_182943   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | bovine, dog, rat, human, pig, horse, rabbit, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | O00469-2 |
| Biochem/physiol Actions: | PLOD2 forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the middle region of human PLOD2 |
| Other Notes: | Synthetic peptide located within the following region: IKGKTLRSEMNERNYFVRDKLDPDM |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |