Anti-NEDD4L antibody produced in rabbit
SIGMA/SAB2103801 - affinity isolated antibody
Synonym: Anti-FLJ33870; Anti-KIAA0439; Anti-RSP5; Anti-hNedd4-2
Product Type: Chemical
Immunoblotting Anti-NEDD4L, antibody produced in rabbit: Cat. No. SAB2103801: Immunoblotting analysis of NEDD4L in PANC1 cell lysate with NEDD4L anitbody at 1 μg/mL.
Western Blotting Western Blot of NEDD4L in Human cystic fibrosis epithelial with NEDD4L antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 110 kDa |
| NCBI accession no. | NM_015277   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | dog, horse, rabbit, rat, human, guinea pig, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q96PU5-5 |
| Biochem/physiol Actions: | NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the middle region of human NEDD4L |
| Other Notes: | Synthetic peptide located within the following region: TVTLSAPLEGAKDSPVRRAVKDTLS |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |